Phenomic and Genomic Characterization of a Mutant Platform in Cucurbita pepo

First TILLING Platform in Cucurbita pepo: A New Mutant Resource for Gene Function and Crop Improvement

Although the availability of genetic and genomic resources for Cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. In this direction, we have developed a high throughput reverse genetic tool: the first TILLING (Targeting Induced Local Lesions IN Genomes) resource for this species. Additionally, we have used this resource to demonstrate that ...

Distribution of a - Galactosidase in Cucurbita pepo

The distribution of a-galactosidase (a-D-galactoside galactohydrolase IEC 3.2.1.221) in Cucurbita pepo has been determined in an attempt to assess its involvement in hydrolysis of transport sugars of the raffinose oligosaccharide series (la-1-6-galactopyranosyllj sucrose). Extracts prepared from leaves and petioles at different stages of development, roots, flowers, dry and germinating seeds, a...

Metabolic Requirement of Cucurbita pepo for Boron.

Lateral roots of intact summer squash seedlings (Cucurbita pepo L.) were used to quantify the effects of boron deficiency on DNA synthesis, protein synthesis, and respiration. The temporal relationship between changes in these metabolic activities and the cessation of root elongation caused by boron deprivation was determined. Transferring 5-day-old squash seedlings to a hydroponic culture medi...

Draft of Zucchini (Cucurbita pepo L.) Proteome: A Resource for Genetic and Genomic Studies

Regulation of Pyrimidine Biosynthesis in Intact Cells of Cucurbita pepo.

The occurrence of the complete orotic acid pathway for the biosynthesis de novo of pyrimidine nucleotides was demonstrated in the intact cells of roots excised from summer squash (Cucurbita pepo L. cv. Early Prolific Straightneck). Evidence that the biosynthesis of pyrimidine nucleotides proceeds via the orotate pathway in C. pepo included: (a) demonstration of the incorporation of [(14)C]NaHCO...